FIGURE 6.

Caspase-2 suppresses NF-κB activation in tumors. Western blotting analysis of protein expression of p-IκBα and NF-κB targeted antiapoptotic proteins as indicated in tumors derived from MEFs deficient in caspase-2 or expressing catalytic dead or S139A mutant caspase-2. A and B, total lysates from day 14 tumor mass derived from each cell lines as indicated were subjected to Western blotting and probed with antibodies recognizing the indicated proteins (A), and the results were quantified using actin as control and normalized to wild-type control by using ImageJ 1.37c (National Institutes of Health) (B). C, immunohistochemical analysis of protein expression of the p65 subunit of NF-κB and NF-κB-targeted antiapoptotic proteins as indicated in formalin-fixed, paraffin-embedded tumor sections derived from each MEF cell lines as indicated.