FIGURE 5.

DMIB head is required for dissociation of DMIB from the plasma membrane, and BH site is required for DMIB localization to cell-cell contacts in randomly moving cells. A, within about 2 h, DMIB (supplemental Movie S2) relocates to cell protrusions whereas Tail remains uniformly concentrated on the plasma membrane (supplemental Movie S3). B, DMIB, dGPQSH3 (supplemental Movie S4), DMIB-S332A, and DMIB-E407K are enriched at random cell-cell contacts, where PIP₂ (PH-PLCδ) also concentrates, and are absent from other regions of the plasma membrane. Tail remains relatively uniformly associated with the plasma membrane and DMIB-BH-Ala, and Head+IQ remains diffused in the cytoplasm. Scale bars, 10 μm.