FIGURE 7.

Localization of DMIB in streaming cells. Images of live streaming cells expressing proteins as marked at the top of the panels are shown. Expressed DMIB colocalizes with PIP₃ (PH-CRAC) at the engulfing mouth of cells in chemotaxing streams as does motor-dead DMIB-S332A and DMIB missing the GPQ and SH3 domains (dGPQSH3). Head+IQ and mutant missing BH site (dGPQSHdBH) are fully cytoplasmic. Expressed Tail and PIP₂ (PH-PLCδ) remain mostly uniformly distributed on the plasma membrane. DMIB point mutant with weakened actin binding (DMIB-E470K) also localizes primarily to the plasma membrane but has a higher cytoplasmic component than does Tail. See also supplemental Movies S8–S11.