FIGURE 2.

Species-specific LeuRS CP1 domain-dependent in vitro splicing of the bI4Δ1168 precursor RNA. The processing of the bI4Δ1168 pre-RNA was evaluated with respect to substrate pre-RNA processing (A), fraction of excised bI4 intron formed (B), and fraction of ligated B4-B5 exons formed (C). Calculations were based on the intensity of the phosphorimaged bands for the substrate and products bI4 and B4-B5 as well as other alternate bands that emerged during the reaction. Splicing reactions incorporated 1 μm pre-RNA and 1 μm LeuRS and were initiated with 1 mm guanosine. ▴, ecCP1-βext; ♦, ymCP1-βext. Error bars for each time point are the result of each reaction repeated in triplicate.