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. 2012 Mar 1;287(18):14772–14781. doi: 10.1074/jbc.M111.322412

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — In vitro splicing activity of isolated CP1 domain-based ymLeuRS Trp-238 mutants. Evaluation of in vitro bI4Δ1168 pre-RNA splicing with respect to processing of substrate pre-RNA (A), fraction of bI4 excised intron (B), and fraction of the ligated B4-B5 exons (C) is shown. Splicing reactions incorporated 1 μm concentrations of substrate pre-RNA and 1 μm LeuRS and were initiated with 1 mm guanosine. ▴, WT ymCP1-βext; ●, W238C ymCP1-βext. Error bars for each time point result from each reaction repeated in triplicate.