TABLE 1.
Basal P2X4HA and P2X2FLAG receptor mobility
| No. of QDs | Corralled D (μm2/s) means ± S.E. (proportion; %) (S.D.) | Linear mobile D (μm2/s) means ± S.E. (proportion; %) (S.D.) | Linear slowly mobile D (×10−4 μm2/s) means ± S.E. (proportion; %) (S.D.) | pa | |
|---|---|---|---|---|---|
| Studies with P2X4HA in microglia | |||||
| Control | 319 | 0.008 ± 0.001 (10) (0.01) | 0.023 ± 0.001 (66) (0.041) | 9.2 ± 3.3 (24) (3.3) | <0.01 |
| + LPSb | 155 | 0.01 ± 0.003 (9) (0.01) | 0.016 ± 0.001 (85) (0.015) | 4.1 ± 1.0 (6) (2.9) | <0.01 |
| + fibronectinb | 68 | 0.017 ± 0.008 (10) (0.02) | 0.012 ± 0.002 (85) (0.011) | 5.0 ± 0.7 (4) (1.2) | <0.01 |
| + LPS + minocycline | 120 | 0.009 ± 0.004 (9) (0.014) | 0.014 ± 0.001 (72) (0.012) | 2.6 ± 0.7 (19) (3.2) | <0.01 |
| + LPS + SB203580 | 130 | 0.007 ± 0.002 (16) (0.007) | 0.011 ± 0.002 (59) (0.013) | 4.6 ± 0.6 (25) (3.4) | <0.01 |
| + poly(I:C) | 55 | 0.012 ± 0.002 (18) (0.008) | 0.015 ± 0.002 (76) (0.013) | 3.3 ± 1.9 (6) (3.3) | <0.01 |
| P2X4HAY378A | 126 | 0.018 ± 0.001 (12) (0.38) | 0.013 ± 0.002 (68) (0.018) | 9.8 ± 6.5 (20) (3.0) | <0.01 |
| + dynasore | 100 | 0.011 ± 0.003 (12) (0.009) | 0.012 ± 0.001 (62) (0.01) | 4.9 ± 0.8 (20) (3.5) | <0.01 |
| + SB203580 | 115 | 0.007 ± 0.002 (9) (0.006) | 0.010 ± 0.002 (53) (0.013) | 3.4 ± 0.5 (38) (3.0) | <0.01 |
| + apyraseb | 70 | 0.002 ± 0.001 (9) (0.002) | 0.022 ± 0.003 (63) (0.02) | 3.6 ± 0.8 (28) (3.6) | <0.01 |
| P2X4HA in HEK293 cells | 181 | 0.007 ± 0.002 (7) (0.01) | 0.018 ± 0.005 (10) (0.022) | 9.59 ± 1.4 (83) (1.8) | <0.01 |
| Studies with P2X2FLAG in microglia | |||||
| P2X2FLAGc | 225 | 0.003 ± 0.001 (12) (0.004) | 0.009 ± 0.001 (48) (0.013) | 2.80 ± 0.39 (40) (4.1) | <0.01 |
| + LPSb + P2X2FLAG | 65 | 0.007 ± 0.001 (11) (0.008) | 0.007 ± 0.002 (37)) (0.007) | 2.40 ± 0.41 (52) (2.4) | <0.01 |
a Paired t tests between linear mobile D and linear lowly mobile D receptors for each experiment are shown.
b LPS, fibronectin, and apyrase treatment did not change the diffusion coefficient for any of the trajectory types (p > 0.05 relative to control with unpaired Student's t tests). However, LPS and fibronectin reduced the proportion of linear mobile P2X4HA receptors relative to P2X4HA under control settings in microglia (Fig. 8). Note that the units of D for the linear slowly mobile pool are scaled by 10−4 as described in the column title.
c P2X2FLAG receptors displayed slower mobility than P2X4HA receptors (p < 0.01) under control conditions in microglia.