FIGURE 5.

H3K9me3 is required for stable association of Lrwd1 and Orc2 with pericentric heterochromatin in vivo. A–C, localization of HP1α (A), Orc2 (B), and Lrwd1 (C) at pericentric heterochromatin in wild-type and Suv420 h1/h2^−/− MEF cells. D–F, localization of HP1α (D), Orc2 (E), and Lrwd1 (F) at pericentric heterochromatin compromised in Suv39 h1/h2^−/− MEF cells. DAPI stains pericentric heterochromatin. Immunofluorescence using antibodies against HP1α, GFP-Lrwd1, or H3K9me3 of wild-type, Suv39 h1/h2^−/−, and Suv420 h1/h2^−/− MEF cells was performed as described under “Experimental Procedures.” Scale bar, 5 μm.