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. 2012 Mar 12;287(18):15054–15065. doi: 10.1074/jbc.M111.337931

FIGURE 2.

FIGURE 2.

Tissue distribution of SNX27 and its interaction with MRP4. A, tissue distribution of SNX27 in humans. A commercially available human multiple-tissue blot was probed with antibodies against SNX27, MRP4, and β-actin. B, tissue distribution of SNX27 in mice. Homogenates prepared from mouse bone marrow, brain, kidney, and liver were subjected to Western blot analysis. C and D, interaction between endogenous MRP4 and SNX27 in mouse bone marrow and kidney (C) and HEK293 cells (D). SNX27 was immunoprecipitated (IP) from tissue homogenates of mouse bone marrow and kidney and lysates of HEK293 cells using anti-SNX27 antibody. Immunoprecipitated specimens were subjected to Western blot analysis (IB). B–D, representative result from three independent experiments.