TABLE 1.
Proteins identified by TbTim17-TAP and mass spectrometry analysis
Proteins identified by at least two peptides are listed. Gene ID numbers are from the GeneDB T. brucei database. Estimated molecular masses and brief descriptions of proteins that have been characterized previously are included. Hypothetical proteins are trypanosome-specific, and no match has been found in other eukaryotes.
| GeneID | Molecular mass | Description | Mitochondriala | TMDb |
|---|---|---|---|---|
| kDa | ||||
| Tb09.160.0670 | 329 | Hypothetical, Hect domain E3 ubiquitin ligase | ||
| Tb927.1.1310 | 47 | Hypothetical, similarity to bacterial outer membrane lipoprotein | + | 2 |
| Tb927.6.2470 | 54 | Hypothetical | + | |
| Tb927.8.1740 | 62.14 | Hypothetical | + | |
| Tb11.01.3290 | 69 | Hypothetical | + | |
| Tb11.02.5660 | 46.2 | Hypothetical, putative complex | + | 4 |
| Tb927.5.2330 | 484.6 | Hypothetical | ||
| Tb927.3.5340 | 42.03 | Hip, Hsc70-interacting protein | 3 | |
| Tb927.10.3620 | 34 | Laminin-like protein | + | 2 |
| Tb927.3.1380 | 55.7 | ATP-synthase β | + | |
| Tb927.5.1060 | 54.1 | MPP, mitochondrial processing peptidase | + | |
| Tb10.70.0430 | 59.5 | Hsp60 | + | |
| Tb927.6.3740 | 71.4 | Hsp70 | + | |
| Tb11.01.3550 | 41.14 | Oxoglutarate dehydrogenase E2 component | + | |
| Tb927.10.2900 | 95.07 | Importin β | ||
| Tb11.47.0004 | 113.39 | Oxoglutarate dehydrogenase E1 component | + | |
| Tb927.8.1420 | 55.9 | Acyl-CoA dehydrogenase IV subunit | + | |
| Tb10.70.5820 | 51.3 | Hexokinase | ||
| Tb09.211.3540 | 56.3 | Glycerol kinase | ||
| Tb927.8.1900 | 25.6 | Tryparedoxin peroxidase |
a Mitochondrial localization of the hypothetical proteins was predicted after analysis by the MitoCarta as well as by the MitoProt program.
b Probable transmembrane domains (TMD) were predicted by TMPred server.