FIGURE 6.
NOESY spectra of ECL2 peptide 2C. A, NOESY spectrum of free 2C. B, transferred NOESY spectrum of 2C in the presence of YU2 gp120. C, expansion of and assignments for cross-peaks in the NH-CαH/CβH region of the spectrum for 2C in complex with gp120. In spectra for the complex, only intraresidue (black labels) and sequential (i to (i+1)) (red labels) correlations were observed; no long range correlations were detected. Spectra were recorded on samples prepared under identical conditions (with concentrations of 800 μm peptide for free 2C, and 800 μm peptide/15 μm gp120 for the complex) and with identical acquisition parameters at 600 MHz; τm = 150 ms.