FIGURE 2.

A and B, overlay of sensorgrams resulting from the injection of different concentrations (10–250 nm) of collagen IV over immobilized GPBP (96 RU; flow rate, 15 μl/min; injected volume, 60 μl) (A) and over immobilized SAP (96 RU; flow rate, 15 μl/min; injected volume, 60 μl) (B). C, binding of collagen IV (100 nm) to immobilized GPBP and CERT (50 μg/ml in 10 mm sodium acetate buffer, pH 4.5). The sensorgrams were corrected for the signal in the empty cell (i.e. calculated as the difference between the signal in Fc2 (flow channel 2) that contained the immobilized ligand and the signal in Fc1 (empty channel), which includes injection noise, instrument drift, and nonspecific binding).