TABLE 1.
Summary of Rpn2-Rpn13, Rpn1-Ubp6, Rpn1-Rad23, and Rpn1-Dsk2 kinetic parameters
Summary of Rpn2-Rpn13, Rpn1-Ubp6, Rpn1-Rad23, and Rpn1-Dsk2 kinetic parameters determined by SPR analysis. All response curves were fitted to a single site binding model (Langmuir kinetics) with the exception of Ubp6 for which the full-length protein required a two-site binding model (2× Ubp6 + Rpn1(site 1) + Rpn1(site 2) ↔ Ubp6-Rpn1(site 1) + Ubp6-Rpn1(site 2)). Values for dissociation constant KD were calculated as the ratio of koff over kon.
| Interaction | kon | koff | KD |
|---|---|---|---|
| m−1s−1 | s−1 | μm | |
| Rpn2-Rpn13 | 1.48 ± 0.31 × 104 | 1.80 ± 0.75 × 10−4 | 0.0118 ± 0.0028 |
| Rpn1-Ubp6 | 1.55 ± 0.36 × 104 | 9.61 ± 3.67 × 10−4 | 0.062 ± 0.004 |
| 3.53 ± 0.73 × 104 | 0.067 ± 0.006 | 1.92 ± 0.26 | |
| Rpn1-Rad23 | 2.02 ± 0.56 × 104 | 0.074 ± 0.022 | 3.65 ± 0.09 |
| Rpn1-Dsk2 | 1.37 ± 0.04 × 104 | 0.165 ± 0.007 | 12.10 ± 0.90 |