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. 2012 Mar 8;287(18):14557–14568. doi: 10.1074/jbc.M112.340570

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Cold ischemia in rat kidneys activates ER stress and generates an angiogenic response. The rats were nephrectomized, and the kidneys were rinsed and incubated in an IGL1® solution for 24 h. A, HERP, PDI, CHOP, ATF4, and sXBP1 transcript levels were measured by qRT-PCR in the rat kidneys after 24 h of cold ischemia, and compared with levels in non ischemic kidneys (n = 4). *, p < 0.05. B, GRP78 and phospho-eIF2α protein levels were measured in the rat kidneys after 24 h of cold ischemia. Left: a representative immunoblot is shown. Right: a densitometric analysis of immunoblots. n = 4. C, VEGFA, bFGF, and ANG protein levels were measured in the rat kidneys after 24 h of cold ischemia. Left: a representative immunoblot is shown. Right: a densitometric analysis of immunoblots, n = 4. D, HIF-1α protein levels were measured in rat kidneys after 24 h of cold ischemia. A representative immunoblot is shown. Hypoxic HREC were used as control.