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. 2012 Mar 6;287(18):14502–14514. doi: 10.1074/jbc.M111.329003

FIGURE 6.

FIGURE 6.

FcγR-mediated phagocytosis induces cADPR formation in J774A.1 murine macrophages. [cADPR]i, and [NAADP]i was measured immediately after the addition of latex beads with or without IgG opsonization using a cycling assay as described under “Experimental Procedures.” A, [cADPR]i increased in a time-dependent manner after initiating FcγR-mediated phagocytosis with IgG-opsonized latex beads. p < 0.01 (*) versus unopsonized latex beads (control LB). B, [NAADP]i increase was not detected after initiating FcγR-mediated phagocytosis with IgG-opsonized latex beads. Each line represents the mean ± S.D. of cADPR or NAADP formation from three independent experiments each.