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S2RPgrmc1 increases NGAL RNA levels. A, RT-PCR for NGAL (top) and actin (bottom) in A549 control (odd lanes) and S2RPgrmc1-knockdown cells (even lanes) at increasing times after serum starvation. The figure is an agarose gel. B, real-time PCR of NGAL cDNA. The sample pattern is the same as in panel A. C, real-time PCR of NGAL in A549 cells infected with a control adenovirus (LacZ) or increasing doses of the Ad-S2R-hbd adenovirus (see Fig. 2A).
