FIGURE 11.

SMAC mimetic AEG40730 sensitizes MDMs to Vpr-induced apoptosis. A, MDMs from three different donors were treated with SMAC mimetic AEG40730 (200 nm) for 24 h, and IAP and Bcl2 expression was evaluated by Western blotting. B and C, following a 24-h treatment with SMAC mimetic, MDMs were treated with Vpr (2.5 μm) for another 24 h. Cells were stained with PI for apoptosis evaluation (B), or they were used for Western blotting to evaluate PARP and caspase-3 cleavage (C). The dot plot in B shows flow cytometry data and the mean percentage of apoptosis ± S.D. (error bars) of six separate experiments with cells from different donors. *, p < 0.05. Western blotting results shown in C are representative of three different donors.