FIGURE 5.

Gβ5S protein is undetectable in the RGS6, RGS7, and RGS11 tKO mouse retina. A, representative immunoblot showing the level of Gβ5L and Gβ5S from WT CTRL, 67dKO, 611dKO, 711dKO, and tKO mouse retinal extracts (15 μg) using anti-Gβ5 (CT215, 1:2,000) and anti-GAPDH (1:50,000) antibodies. B, quantification of Gβ5L and Gβ5S levels, normalized to GAPDH signal. Retinal Gβ5L protein levels do not differ across the various genotypes. However, 67dKO contains ∼5% of normal Gβ5S protein level (n = 3), 611dKO contains ∼35% of normal Gβ5S protein level (n = 3), 711dKO contains ∼60% of normal Gβ5S protein level (n = 3), and tKO has undetectable Gβ5S protein level (n = 3). Error bars are S.E.