Table 2.
The physiology and enzymatic properties of Z1EX0 series strains
| Strain | Xylanases expressed | Max OD600 | Protein secretion (g/l) | Endoxylanase activity | Xylosidase activity | |||
|---|---|---|---|---|---|---|---|---|
| 8 h(16 h)a | 24 h | (U/L) | (U/mg) | (U/L) | (mU/mg) | |||
| Bl21(DE3) | - | 6.50 ± 0.17 | 0.03 ± 0 | 0.03 ± 0 | n.d.b | n.d.b | n.d.b | n.d.b |
| Z1000 | - | 6.19 ± 0.08 | 0.04 ± 0 | 0.03 ± 0 | n.d.b | n.d.b | n.d.b | n.d.b |
| Z1140 | E1, X4 | 3.45 ± 0.04 | 0.30 ± 0.02 | 0.69 ± 0 | 398 ± 13 | 1.31 ± 0.07 | 24 ± 1.0 | 80 ± 4 |
| Z1150 | E1, X5 | 4.08 ± 0.12 | 0.19 ± 0.01 | 0.31 ± 0.03 | 65 ± 2 | 0.34 ± 0.01 | 14.3 ± 0.6 | 75 ± 4 |
| Z1160 | E1, X6 | 4.55 ± 0.05 | 0.10 ± 0.01 | 0.21 ± 0.02 | 75 ± 0 | 0.75 ± 0.04 | 6.1 ± 0.3 | 61 ± 5 |
| Z1170 | E1, X7 | 2.02 ± 0.05 | 0.60 ± 0.04 | 0.95 ± 0.03 | 420 ± 19 | 0.70 ± 0.04 | 46.3 ± 0.5 | 77 ± 3 |
| Z1240 | E2, X4 | 5.12 ± 0.12 | 0.10 ± 0.01 | 0.17 ± 0.01 | 274 ± 10 | 2.71 ± 0.19 | 8.0 ± 0.3 | 77 ± 6 |
| Z1250 | E2, X5 | 5.69 ± 0.29 | 0.11 ± 0.02 | 0.12 ± 0.03 | 50 ± 4 | 0.47 ± 0.03 | 6.8 ± 0.2 | 63 ± 7 |
| Z1260 | E2, X6 | 4.83 ± 0.05 | 0.07 ± 0 | 0.10 ± 0 | 90 ± 5 | 1.36 ± 0.04 | 4.5 ± 0.2 | 64 ± 1 |
| Z1270 | E2, X7 | 4.07 ± 0.05 | 0.34 ± 0.01 | 0.43 ± 0.02 | 423 ± 14 | 1.25 ± 0.04 | 29.3 ± 1.2 | 86 ± 3 |
| Z1340 | E3, X4 | 5.10 ± 0.03 | 0.08 ± 0.01 | 0.21 ± 0.01 | 1098 ± 28 | 13.22 ± 1.05 | 7.3 ± 0.2 | 88 ± 7 |
| Z1350 | E3, X5 | 3.97 ± 0.09 | 0.22 ± 0 | 0.51 ± 0.04 | 1512 ± 2 | 6.88 ± 0 | 10.9 ± 0.5 | 49 ± 1 |
| Z1360 | E3, X6 | 4.93 ± 0.10 | 0.08 ± 0 | 0.25 ± 0 | 996 ± 1 | 13.14 ± 0.01 | 5.9 ± 0.2 | 77 ± 1 |
| Z1370 | E3, X7 | 5.04 ± 0.05 | 0.18 ± 0.01 | 0.25 ± 0.01 | 2151 ± 14 | 12.14 ± 0.34 | 16.3 ± 0.2 | 92 ± 3 |
a Except maximum OD600, the sampling time was at the late-exponential phase, which was at about 8 h after induction for strains Z13X0 and the two controls, and 16 h for the other strains.
b n.d.: not detectable.