Table 4.
The physiology and enzymatic properties of the engineering strains harboring a p15A-devirative plasmid
| Strain | Max. OD600 | Protein (g/l) | Endoxylanase activity | Xylosidase activity | |||
|---|---|---|---|---|---|---|---|
| Intracellular | Extracellular | (U/L) | (U/mg) | (U/L) | (mU/mg) | ||
| Z3370 | 5.49 ± 0.06 | 0.53 ± 0.03 | 0.16 ± 0.01 | 1945 ± 60 | 12.48 ± 0.57 | 15.0 ± 0.3 | 94 ± 4 |
| Z3371 | 2.86 ± 0.05 | 0.13 ± 0.02 | 0.32 ± 0.01 | 2022 ± 16 | 6.32 ± 0.12 | 22.7 ± 0.3 | 71 ± 2 |
| Z4370 | 5.75 ± 0.10 | 0.44 ± 0.02 | 0.18 ± 0.01 | 2581 ± 97 | 14.34 ± 0.67 | 17.0 ± 0.2 | 103 ± 3 |
Except maximum OD600, the sampling time was at the late-exponential phase, which was at about 8 h for the three strains.