Table 5.
Strains and plasmids used in this study
| Strain | Genotype | Source |
|---|---|---|
| E. coli W1485 | F+ rpoS396(Am) rph-1 | Lab stocked |
| E. coli BL21 (DE3) | F' ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1ind1 sam7 nin5]) | Novagen |
| E. coli TOP10 | F' mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZ ΔM15 ΔlacΧ74 recA1 araD139Δ(ara-leu) 7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| Z0001 | BL21 (DE3): Δlpp | This study |
| Z0003 | W1485: ΔldhA, ΔptsG::cat, ΔpflB::kan | This study |
| Z1000 | BL21 (DE3): pTrc99z | This study |
| Z1EX0 | BL21 (DE3): pA-EmXn | This study |
| Z1371 | Z0001: pA-E3X7 | This study |
| Z2370 | BL21 (DE3): pA-E3X7D | This study |
| Z3000 | BL21 (DE3): pTrc15a | This study |
| Z3370 | BL21 (DE3): pB-E3X7 | This study |
| Z3371 | Z0001: pB-E3X7 | This study |
| Z4370 | BL21 (DE3): pB-E3X7D | This study |
| Z5370 | BL21 (DE3): pB-E3X7AD | This study |
| Z6373 | Z0003: pB-E3X7AYD | This study |
| Plasmid | Description | Source |
| pUC57-Em | pMB1 ori, Plac : endoxylanase gene (E1, E2 or E3 gene), Ampr | Genscript |
| pUC57-Xn | pMB1 ori, Plac: xylosidase gene (X4, X5, X6 or X7 gene), Ampr | Genscript |
| pUC57-EmXn series | pMB1 ori, Plac:osmY-endoxylanase Em, osmY-xylosidase Xn, Ampr | This study |
| pTrc99a | pBR322 ori, Ampr | Invitrogen |
| pTrc99z | pBR322 ori, Ampr | This study |
| pA-EmXn series | pBR322 ori, Ptrc:osmY-endoxylanase Em, osmY-xylosidase Xn, Ampr | This study |
| pA-E3X7D | pBR322 ori, Ptrc: osmY-xynC-A, osmY-xyloA; PdsbA: dsbA, Ampr | This study |
| pACYC184 | p15a ori, Catr Tetr | NEB |
| pTrc15a | p15a ori, Ampr | This study |
| pB-E3X7 | p15a ori, Ptrc: osmY-xynC-A, osmY-xyloA, Ampr | This study |
| pB-E3X7D | p15a ori, Ptrc: osmY-xynC-A, osmY-xyloA; PdsbA: dsbA, Ampr | This study |
| pB-E3X7OD | p15a ori, Ptrc: osmY-xynC-A, osmY-xyloA, osmY; PdsbA: dsbA, Ampr | This study |
| pB-E3X7AD | p15a ori, Ptrc: osmY-xynC-A, osmY-xyloA, osmY-abf2; PdsbA: dsbA, Ampr | This study |
| pB-E3X7AYD | p15a ori, Ptrc: osmY-xynC-A, osmY-xyloA, osmY-abf2; Ptrc: pyc; PdsbA: dsbA, Ampr | This study |