Table 4.
Primers used in this study for gene cloning and plasmid construction
| Oligonucleotides | Sequences, 5'-3' | Usage |
|---|---|---|
| F-RoFUM1 | ATGTTGCGAGCTTCTGCTACC | Cloning of RoFUM1 |
| R-RoFUM1 | TTAATCCTTGGCAGAGATCATATCTT | |
| F-RoMDH | ATGTTTGCCGCCTCTCGTG | Cloning of RoMDH |
| R-RoMDH | TTATTGAACAAAGCTGTTACCCTTG | |
| BamHI-F(RoMDH) | CGGGATCCATGTTTGCCGCCTCTCGTG | Construction of pY26TEF-GPD-RoMDH |
| HindIII-R(RoMDH) | CCCAAGCTTTTATTGAACAAAGCTGTTACCCTTG | |
| BamHI-F(MDH3ΔSKL) | CGGGATCCATGGTCAAAGTCGCAATTCTTG | Construction of pY26TEF-GPD- MDH3ΔSKL |
| HindIII-R(MDH3ΔSKL) | CCCAAGCTTTCAAGAGTCTAGGATGAAACTCTTGCCT | |
| NotI-F(RoFUM1) | ATAAGAATGCGGCCGCATGTTGCGAGCTTCTGCTACC | Construction of pY26TEF- RoFUM1-GPD- RoMDH |
| BglII-R(RoFUM1) | GAAGATCTTTAATCCTTGGCAGAGATCATATCTT | |
| PstI -F(PYC2) | AACTGCAGATGAGCAGTAGCAAGAAATTGGC | Construction of pRS305TEF1-PYC2 |
| SalI -R(PYC2) | ACGCGTCGACTTACTTTTTTTGGGATGGGGGT |
Abbreviations: F, forward; R, reverse. Underlined sequences indicate the recognition sites for the indicated restriction enzymes.