Table 2.
Activation of promoters Pchs_H1 and Pchs4 with selected Myb and hop TFs in various combinations
| Myb (M) | AC | M | MB* | MW** | MB*W** |
|---|---|---|---|---|---|
| Pchs_H1 | |||||
| HlMyb2 | FN646081 | (+) | + | (+) | ++ |
| s-HlMyb3 | AM501509 | 0 | + | 0 | ++ |
| l-HlMyb3 | AM501509 | 0 | + | 0 | 0 |
| HlMyb1# | AJ876882 | 0 | (+) | n.d. | (++) |
| HlMyb7 | AB292244 | 0 | 0 | 0 | 0 |
| AtMyb23 | NM_123397 | (+) | (+) | + | ++ |
| AtMyb12 | NM_130314 | ++ | ++ | ++ | 0 |
| AtMyb75# | NM_104541 | + | + | + | ++ |
| Pchs4 | |||||
| HlMyb2 | FN646081 | 0 | 0 | 0 | (+) |
| s-HlMyb3 | AM501509 | + | + | + | 0 |
| l-HlMyb3 | AM501509 | + | (++) | 0 | 0 |
| HlMyb7 | AB292244 | 0 | 0 | 0 | 0 |
| AtMyb23 | NM_123397 | 0 | 0 | (+) | 0 |
| AtMyb12 | NM_130314 | + | 0 | + | 0 |
| AtMyb75$ | NM_104541 | 0 | (+) | 0 | (+) |
* B = HlbHLH2 (AC: FR751553)
**W = HlWD40_1 (AC: FN989721)
$ AtPAP1;
#not tested for Pchs4
n.d. not determined
(+) up to 10% of maximal level
+ up to 70% of maximal level
++ maximum activity observed; (++) low maximal activity; the maximal levels for M/promoter/combination/pmol GUS/mg/min: HlMyb2/PchsH1/MBW/5127; s-HlMyb3/PchsH1/MBW/1192; l-HlMyb3/Pchs4/MB/511; HlMyb1/Pchs_H1/MBW/304; AtMyb23/P_chsH1/MBW/2036; AtMyb12/P_chsH1/MW/3108; AtMyb75/Pchs_H1/MBW/5786
0 = no increase of GUS activity over the background caused by M; no enhancement caused by MB or MW over M; no enhancement caused by MBW over M or over the binary combinations.