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. Author manuscript; available in PMC: 2013 Apr 18.
Published in final edited form as: J Am Chem Soc. 2012 Apr 4;134(15):6855–6864. doi: 10.1021/ja301255n

Figure 2.

Figure 2

(1–109)MDM2 K36C/pS17 characterized by RP-HPLC and ESI-MS. The chromatogram was obtained at 40 °C on a Waters XBridge C18 column (4.6×150 mm, 3.5 µm) running a gradient of 5% to 65% acetonitrile in water, containing 0.1% TFA, at a flow rate of 1 ml/min. The determined molecular mass of 12551.0 Da is with experimental error of the theoretical value of 12551.6 Da calculated on the basis of the average isotopic compositions of (1–109)MDM2 K36C/pS17.