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. Author manuscript; available in PMC: 2013 Apr 18.
Published in final edited form as: J Am Chem Soc. 2012 Apr 4;134(15):6855–6864. doi: 10.1021/ja301255n

Figure 3.

Figure 3

Binding of N-acetyl-(15–29)p53-FAM to (25–109)MDM2 and (25–109)MDM2 K36C as quantified by fluorescence polarization techniques at room temperature in 10 mM Tris-HCl buffer containing 150 mM NaCl and 1 mM EDTA, pH 7.0. The fluorescently labeled p53 peptide at 10 nM was incubated with varying concentratiuons of MDM2 for 30 min on a 96-well plate before polarization measurements (λex = 470 nm, λem = 530 nm). The Kd values (mean ± SEM, n=3) are from three independent experiments.