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. Author manuscript; available in PMC: 2013 Apr 18.
Published in final edited form as: J Am Chem Soc. 2012 Apr 4;134(15):6855–6864. doi: 10.1021/ja301255n

Figure 7.

Figure 7

(A) Quantification of the interaction of (1–109)MDM2 pS17 (250 nM) with varying concentrations of Nutlin-3a, (15–29)p53, (17–28)p53 and (19–26)p53 by the SPR-based competitive binding assay described in the legend of Figure 5. Each curve is the mean of three independent measurements, and the Kd values (mean ± SEM, n=2) are listed in Table 1. (B) Binding of N-acetyl-(15–29)p53-FAM to (1–109)MDM2, (1–109)MDM2 S17D, and (1–109)MDM2 pS17 as quantified by the fluorescence polarization assay described in the legend of Figure 3. Each curve is the mean for a representative assay performed in triplicate, and the Kd values (mean ± SEM, n=3) are from three independent assays.