Skip to main content
. 2012 Apr 30;7(4):e36247. doi: 10.1371/journal.pone.0036247

Figure 5. Differentiation of THP-1 cells by M. genitalium strains.

Figure 5

A. Adherent THP-1 cells showing fluorescence. CFSE labeled THP-1 cells were infected with M. genitalium strains (MOI 1∶5). Images of adherent cells were acquired using confocal laser scanning microscope with 10× objective and 488 nm laser. G37, MS5 and MGRE are wild type, msrA mutant and control M. genitalium strains respectively. HKG37 represents heat killed bacteria of wild type M. genitalium. B. Graph showing the amount of adherent cells for each infection. The number of labeled cells in each image were counted using the particle plugin of Image J software. Average cell numbers from five different optical fields and from three independent experiments were used for determining the number of adherent mononuclear cells in each infection. Labels are as described in “A”. * = p≤0.05 vs wild type G37 strain.