Skip to main content
. 2012 Apr 30;7(4):e36389. doi: 10.1371/journal.pone.0036389

Figure 1. HOP promotes endothelial tube formation and sprouting.

Figure 1

A. HUVECs transfected with HOP or control siRNAs were plated onto matrigel, and photographs were taken 4 and 16 hours later. B. Immunoblotting analysis of HOP and β-actin expression in HUVECs transfected with HOP or control siRNAs. C. Experiments were performed as in A, and the cumulative tube length was measured. *, p<0.05 versus control. D. HUVECs transfected with GFP, GFP-HOP, GFP-HOP plus HOP shRNA, or GFP-mHOP (shRNA-resistant mutant) plus HOP shRNA were plated onto matrigel, and photographs were taken 1, 3, and 6 hours later. E. Experiments were performed as in D, and the cumulative tube length was measured. *, p<0.05. F. Capillary-like sprout formation from spheroids generated from HUVECs transfected with HOP or control siRNAs. G. Experiments were performed as in F, and the cumulative sprout length was measured. *, p<0.05 versus control.