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. 2012 Apr 30;7(4):e36389. doi: 10.1371/journal.pone.0036389

Figure 2. Knockdown of HOP expression does not affect the proliferative behavior or apoptotic rate of vascular endothelial cells.

Figure 2

A. Immunoblotting analysis of HOP and β-actin expression in HUVECs transfected with HOP shRNA or the control vector for 96 hours. B. Growth curves of HUVECs transfected with HOP shRNA or the control vector, measured by sulforhodamine B staining assay. C. HUVECs transfected with HOP shRNA or the control vector for 48 or 96 hours were stained with fluorescein-conjugated annexin V. Phase contrast and fluorescence images were then taken. D. Experiments were performed as in C, and the percentage of apoptotic cells was then quantified.