Figure 6. The inhibitory effect of Uif* is dependent on the extracellular domain of Notch.

Ectopic expression of the full-length Notch (N^FL) under the control of dpp-Gal4 induces aberrant Wg (red) expression at the AP boundary where it intersects with the DV boundary (white arrow in A) (A and A′). GFP (green) marks dpp-Gal4 positive cells (A′, B′, C′ and D′). Coexpression of Uif* with N^FL reduces the ectopic induction of Notch signaling mediated by N^FL at the intersection between AP and DV boundaries (white arrow in B) (B and B′). Ectopic expression of the membrane tethered active version of Notch (N^ECN) induces Wg (red) expression in the dpp-Gal4 region that is marked by GFP (green) (C and C′). Coexpression of Uif* does not alter the Wg expression that is induced by N^ECN (D and D′).