Table 1. Similarity-based OTUs and species richness estimates at 3%, 5% and 10% dissimilarity levels.
OTUs | ACE | Chao1 | OTUs | ACE | Chao1 | ||||||||||
2008 | First time | non-Bt | RDP | 0.03 | 1148 | NC | 2480±279 | 2010 | First time | non-Bt | RDP | 0.03 | 952 | NC | 2126±266 |
0.05 | 846 | NC | 1608±164 | 0.05 | 765 | NC | 1429±172 | ||||||||
0.1 | 527 | NC | 670±49 | 0.1 | 465 | NC | 668±77 | ||||||||
ESPRIT | 0.03 | 1031 | 1842.63 | 1895±194 | ESPRIT | 0.03 | 916 | 2117.53 | 1894±227 | ||||||
0.05 | 728 | 1110.54 | 1065±98 | 0.05 | 745 | 1531.48 | 1430±182 | ||||||||
0.1 | 316 | 341.97 | 333±14 | 0.1 | 433 | 568.97 | 534±44 | ||||||||
Bt | RDP | 0.03 | 1135 | NC | 2469±272 | Bt | RDP | 0.03 | 920 | NC | 2199±297 | ||||
0.05 | 918 | NC | 1528±173 | 0.05 | 711 | NC | 1289±156 | ||||||||
0.1 | 541 | NC | 745±81 | 0.1 | 425 | NC | 592±69 | ||||||||
ESPRIT | 0.03 | 1035 | 2075 | 2009±215 | ESPRIT | 0.03 | 886 | 2228.54 | 1970±259 | ||||||
0.05 | 795 | 1309.08 | 1269±126 | 0.05 | 692 | 1315.88 | 1179±136 | ||||||||
0.1 | 447 | 549.7 | 540±42 | 0.1 | 406 | 543.15 | 529±54 | ||||||||
Final time | non-Bt | RDP | 0.03 | 1053 | NC | 2820±406 | Final time | non-Bt | RDP | 0.03 | 1011 | NC | 2414±311 | ||
0.05 | 797 | NC | 1687±211 | 0.05 | 815 | NC | 1595±196 | ||||||||
0.1 | 498 | NC | 703±50 | 0.1 | 537 | NC | 807±94 | ||||||||
ESPRIT | 0.03 | 982 | 1951.57 | 2087±260 | ESPRIT | 0.03 | 1010 | 2508.72 | 2201±260 | ||||||
0.05 | 755 | 1247.91 | 1303±153 | 0.05 | 805 | 1556.53 | 1384±146 | ||||||||
0.1 | 415 | 502.03 | 506±44 | 0.1 | 469 | 642.18 | 630±65 | ||||||||
Bt | RDP | 0.03 | 1216 | NC | 2230±258 | Bt | RDP | 0.03 | 999 | NC | 2420±317 | ||||
0.05 | 963 | NC | 1474±179 | 0.05 | 819 | NC | 1783±243 | ||||||||
0.1 | 568 | NC | 679±71 | 0.1 | 493 | NC | 688±73 | ||||||||
ESPRIT | 0.03 | 1029 | 1992.92 | 1947±204 | ESPRIT | 0.03 | 1015 | 2679.5 | 2341±293 | ||||||
0.05 | 726 | 1095.15 | 1013±84 | 0.05 | 821 | 1692.52 | 1596±196 | ||||||||
0.1 | 326 | 352.58 | 342±14 | 0.1 | 485 | 632.34 | 609±51 | ||||||||
2009 | First time | non-Bt | RDP | 0.03 | 1307 | NC | 2565±350 | 2011 | First time | non-Bt | RDP | 0.03 | 898 | NC | 2138±296 |
0.05 | 1033 | NC | 1661±204 | 0.05 | 691 | NC | 1300±171 | ||||||||
0.1 | 608 | NC | 663±85 | 0.1 | 429 | NC | 606±71 | ||||||||
ESPRIT | 0.03 | 1105 | 2395.43 | 2287±249 | ESPRIT | 0.03 | 847 | 1916.25 | 1774±228 | ||||||
0.05 | 865 | 1583.68 | 1602±186 | 0.05 | 680 | 1265.76 | 1204±149 | ||||||||
0.1 | 473 | 602.51 | 593±51 | 0.1 | 398 | 523.2 | 517±53 | ||||||||
Bt | RDP | 0.03 | 1184 | NC | 2454±253 | Bt | RDP | 0.03 | 884 | NC | 2253±332 | ||||
0.05 | 910 | NC | 1650±178 | 0.05 | 690 | NC | 1430±208 | ||||||||
0.1 | 503 | NC | 654±60 | 0.1 | 407 | NC | 540±58 | ||||||||
ESPRIT | 0.03 | 1104 | 2315.27 | 2303±255 | ESPRIT | 0.03 | 852 | 1990.86 | 2068±609 | ||||||
0.05 | 847 | 1445.27 | 1448±154 | 0.05 | 651 | 1178.58 | 1194±160 | ||||||||
0.1 | 470 | 574.01 | 556±39 | 0.1 | 360 | 441.06 | 429±35 | ||||||||
Final time | non-Bt | RDP | 0.03 | 1252 | NC | 3070±395 | Final time | non-Bt | RDP | 0.03 | 902 | NC | 2068±273 | ||
0.05 | 1010 | NC | 1897±241 | 0.05 | 717 | NC | 1318±161 | ||||||||
0.1 | 596 | NC | 819±76 | 0.1 | 411 | NC | 512±53 | ||||||||
ESPRIT | 0.03 | 1063 | 2108.05 | 1899±179 | ESPRIT | 0.03 | 853 | 1961.8 | 1730±213 | ||||||
0.05 | 770 | 1266.42 | 1234±128 | 0.05 | 675 | 1235.8 | 1095±119 | ||||||||
0.1 | 362 | 397.08 | 387±18 | 0.1 | 370 | 460.93 | 433±32 | ||||||||
Bt | RDP | 0.03 | 1204 | NC | 2730±301 | Bt | RDP | 0.03 | 868 | NC | 2184±326 | ||||
0.05 | 904 | NC | 1636±176 | 0.05 | 665 | NC | 1208±155 | ||||||||
0.1 | 539 | NC | 728±71 | 0.1 | 380 | NC | 529±65 | ||||||||
ESPRIT | 0.03 | 996 | 1808.36 | 1810±186 | ESPRIT | 0.03 | 828 | 1925.67 | 1793±242 | ||||||
0.05 | 716 | 1112.28 | 1062±101 | 0.05 | 648 | 1211 | 1153±149 | ||||||||
0.1 | 338 | 367.01 | 354±13 | 0.1 | 357 | 456.86 | 444±43 |
The species richness estimates were determined using the RDP pyrosequencing pipeline or the ESPRIT program, as described in Materials and Methods. NC = non computable.