Table 2. Multiplex identifiers (MIDs).

Year	Samplig time	MID Bt-maize	MID Non Bt-maize
2008	First	MID1 (5′-ACGAGTGCGT-3′)	MID2 (5′- ACGCTCGACA -3′)
	Final	MID3 (5′- AGACGCACTC -3′)	MID4 (5′- AGCACTGTAG -3′)
2009	First	MID5 (5′-ATCAGACACG-3′)	MID6 (5′- CGTGTCTCTA -3′)
	Final	MID7 (5′- CGTGTCTCTA -3′)	MID8 (5′- CTCGCGTGTC -3′)
2010	First	MID1 (5′-ACGAGTGCGT-3′)	MID2 (5′- ACGCTCGACA -3′)
	Final	MID3 (5′- AGACGCACTC -3′)	MID4 (5′- AGCACTGTAG -3′)
2011	First	MID1 (5′-ACGAGTGCGT-3′)	MID2 (5′- ACGCTCGACA -3′)
	Final	MID3 (5′- AGACGCACTC -3′)	MID4 (5′- AGCACTGTAG -3′)

The Multiplex identifiers (MIDs) used for pyrosequencing the different samples are shown.