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. 2012 Apr 30;7(4):e35924. doi: 10.1371/journal.pone.0035924

Figure 6. FXR agonist, GW-4064, reverses the effects exerted by p17 on THP-1 cells.

Figure 6

THP-1 cells were pre-incubated 2 hours with GW-4064 (1 µM) before administration of p17 (1 µg/ml for 18 hours). Total RNA was extracted and the relative mRNA expression of (A) proinflammatory cytokines and nuclear receptors, (B) co-stimulatory molecules and (C) proatherogenic genes was assayed by Real-Time PCR. Values are expressed relative to not treated cells. Analysis was carried out in triplicate and the experiment was repeated twice. *P<0.05 versus not treated cells. **P<0.05 versus p17 stimulated cells.