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. 2012 Apr 30;7(4):e36084. doi: 10.1371/journal.pone.0036084

Figure 2. Standard curve titration of ASLNAqPCR for KRAS.

Figure 2

Serial dilution of the KRAS G12V mutated SW620 cell line DNA in wild type DNA. Gray squares correspond to 50%, 20%, 10%, 5%, 1%, 0,1%, 0.01% mutant to wild type DNA ratios (duplicate samples). The titration slope is −3.076, R2 is 0.991 (top right), corresponding to a PCR efficiency of 111.3%.