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Serial dilution of the BRAF V600E mutated OCUT cell line DNA in wild type DNA. Gray squares correspond to 50%, 20%, 10%, 5%, 1%, 0,1%, 0.01% mutant to wild type DNA ratios (duplicate samples). The titration slope is −2.985, R2 is 0.991 (top right), corresponding to a PCR efficiency of 116.2%.
