Table 3. Primers and beacon probes.
Sanger Sequencing | |||
Gene | Exon | Forward Primer | Reverse Primer |
KRAS | 2 | AAGGTGAGTTTGTATTAAAAGGTACTGG | TGGTCCTGCACCAGTAATATGC |
3 | TCCAGACTGTGTTTCTCCCTTCTC | AAAACTATAATTACTCCTTAATGTCAGCTT | |
BRAF | 15 | TCATAATGCTTGCTCTGATAGGA | GGCCAAAAATTTAATCAGTGGA |
bp, base pair. “+” precedes LNA-modified nucleotides.