Table 5. Frequence of specific KRAS and BRAF mutations cases analyzed by SSEQ and ASLNAqPCR.
| Gene | Mutation | SSEQ (%) | ASLNA (%) |
| KRAS (n = 300) | G12D | 41/274 (14.9) | 48/286 (16.8) |
| G12V | 23/274 (8.4) | 26/286 (9.1) | |
| G13D | 17/274 (6.2) | 19/286 (6.6) | |
| G12C | 8/274 (2.9) | 12/286 (4.2) | |
| G12S | 3/274 (1.1) | 4/286 (1.4) | |
| G12A | 3/274 (1.1) | 3/286 (1.1) | |
| G12R | 2/274 (0.7) | 5/286 (1.7) | |
| G12F | 1/274 (0.4) | NT | |
| Q61H | 3/274 (1.1) | NT | |
| Q61L | 2/274 (0.7) | NT | |
| All mutant cases | 103/274 (37.6) | 117/286 (40.9) | |
| No amplifiable DNA | 26/300 (8.7) | 14/300 (4.7) | |
| BRAF (n = 201) | V600E | 15/194 (7.7) | 20/194 (10.3) |
| No amplifiable DNA | 7/201 (3.5) | 7/201 (3.5) |
SSEQ, Sanger sequencing; ASLNA, allele specific quantitative PCR using 3′-locked nucleic acid modified primers (ASLNAqPCR); NT, not tested, since ASLNAqPCR primers were designed to identify only the seven most common codon 12−13 KRAS mutations.