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Outline of the genetic system used to obtain mutator mutants of E. coli carrying defects in the nrdAB genes. Plasmid pHABcat is mutagenized (*) in vitro with hydroxylamine and used to transform a strain containing plasmid pHABamp and a chromosomal nrdAB deletion. Replacement of pHABamp by pHABcat then allows scoring for a mutator phenotype based on colony papillation (lacZ reversion). See text and Materials and Methods for details.
