Table 1.
Large animal iPSC derivation overview
| Date and author | Parental cell source | Feeder layer | Special culture conditions | Differentiation (in vitro) | Differentiation (in vivo) | Markers | Reprogramming factors | |
|---|---|---|---|---|---|---|---|---|
| Canine iPSCs | 2009 Shimada et al. | Canine embryonic fibroblasts | MEFs | 6 ng/ml hbFGF, 1000 U/ml hLIF, 1 mM VPA, 0.5 mM PD0325901, 3 mM CHIR99021, 0.25 mM A83-01 | AFP, FLK1, Beta III-tubulin positive cells | None | AP, Oct3/4 | Retrovirus Canine OKSM |
| 2011 Lee et al. | Canine fibroblasts, Canine adipose stromal cells | MEFs | 5 ng/ml hbFGF, 1000 U/ml hLIF | Embryoid bodies, Endothelial cells | Teratomas | AP, Oct3/4, Sox2, Nanog, TRA- 1-60, and SSEA-4 | Lentivirus Human OKSM | |
| Porcine iPSCs | 2009 Ezashi et al. | Porcine foetal fibroblasts | MEFs | 4 ng/ml hbFGF | Embryoid Bodies | Teratomas | AP, SSEA-1, Oct3/4, Nanog, Sox2 | Lentivirus Human OKSM |
| 2009 Esteban et al | Tibetan miniature pig fibroblast | MEFs | 4 ng/ml hbFGF, mLIF, PD0325901, CHIR99021, 39°C | None | Teratomas | AP, Nanog, Sox2, Klf4, Oct3/4, Rex1, Lin28, SSEA4 | Retrovirus Mouse and Human OKSM | |
| 2009 Wu et al. | Primary ear fibroblast and primary bone marrow cells | MEFs | DMEM/F12 + 20% KSR + DOX | Embryoid bodies | Teratomas | AP, SSEA3, SSEA4, Tra-1-60, Tra-1-81, Oct3/4, Nanog, Sox2, Rex1 and CDH1. | DOX inducible Lentivirus Human OKSM + Nanog, Lin28 | |
| 2010 West et al. | Porcine mesenchymal stem cells | 1. MEFs 2. Matrigel | 1. DMEM + 20% KSR + 10 ng/ml hbFGF 2. mTeSR1 | Embryoid bodies | Chimeric offspring | AP, Oct3/4, Sox2, | Lentivirus Human OKSM + NANOG, LIN28 | |
| 2010 Montserrat et al. | Pig ear fibroblasts | MEFs or Gelatin only | hES/mES media (1:1) 10 ng/ml hbFGF, 1000 U/ml LIF | Embryoid bodies | Teratomas | Nanog, SSEA-4, TRA-1-60 | Retrovirus pMXs–OKSM–GFP | |
| Sheep iPSCs | 2010 Li et al. | Sheep foetus fibroblast | MEFs | FBS instead of KSR | Embryoid bodies | Teratomas | AP, Oct3/4, Sox2, Nanog, SSEA-4 | DOX inducible lentivirus Mouse OKSM |
| Boving iPSCs | 2011 Sumer et al. | Bovine adult fibroblast | MEFs | 10 ng/ml bovine bFGF, 4 ng/ml hLIF, 39°C | Embryoid bodies | Teratomas | AP, Rex1, Oxt3/4, Sox2, Nanog, c-Myc, Klf4, SSEA1, SSEA4 | Retrovirus OKSM + Nanog |
| Non-human primate iPSCs | 2008 Liu et al. | Adult rhesus macaque ear fibroblast | MEFs | KSR medium | Embryoid bodies | Teratomas | AP, Nanog, Oct3/4, Sox2, SSEA4, TRA-1-60, TRA-1-81 | Retrovirus Monkey OKSM |
| 2010 Zhong et al. | Macaca nemestrina oral fibroblasts | MEFs | 16 ng/ml hbFGF, 0.5 mM PD0325901, 2 mM VPA, and 10 mM Y-27632 | Embryoid bodies Neural precursors, Cardiomyocytes, Hepatocytes | Teratomas | Oct3/4, SSEA3, SSEA4, TRA-1-60, TRA-1-81, Nanog | Retrovirus OKSM | |
| 2011 Deleidi et al. | Cynomolgus macaque skin fibroblasts | MEFs | KSR medium | Neurons | Teratomas | AP, Nanog, Oct3/4, Sox2, Klf4, cMyc, TRA-1-60, TRA-1-81, SSEA4 | Retrovirus hOKSM |
AP: alkaline phosphatase; bFGF: basic fibroblast growth factor; FBS: foetal bovine serum; KSR: knockout serum replacement; LIF: leukaemia inhibitory factor; MEF: mouse embryonic fibroblasts; VPA: valproic acid; MEF: mouse embryonic fibroblast.