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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: DNA Repair (Amst). 2012 Apr 6;11(5):488–501. doi: 10.1016/j.dnarep.2012.02.004

Fig. 4.

Fig. 4

The PGBD3 piggyBac transposase and the CSB-PGBD3 fusion protein bind to consensus MER85 elements in vitro. Electrophoretic mobility shift assays were performed [25] for binding of the recombinant PGBD3 and CSB-PGBD3 fusion proteins to 6 different genomic MER85 elements that closely match the 140 bp Repbase MER85 consensus. The multiple sequence alignment and genomic primers are shown in Fig. S2. Chromosome number is indicated above the lanes. MER85 elements from chromosomes 1 and 7 are MseI/MseI and AseI/HincII fragments, respectively, lacking flanking sequence; MER85s from chromosomes 2, 6, 8, and 17 are BamHI/EcoRI fragments that include the flanks. Although both proteins shift efficiently, the piggyBac transposase generates sharper bandshifts than the larger fusion protein on low percentage gels (6% 29:1 acrylamide:bisacrylamide for PGBD3 and 5% 80:1 acrylamide:bisacrylamide for CSB-PGBD3).