Table 1. Cell lines used in this and the previous study.

The primary GM00739 fibroblast line derived from patient CS1AN was obtained from the Coriell Institute and transformed with retroviral hTERT [3]. UVSS1KO fibroblasts derived from patient UVSS1KO and transformed with a replication-defective SV40 [13] were the kind gift of Kiyoji Tanaka (Osaka University). EGFP, enhanced green fluorescent protein; hyg, hygromycin resistance; neo, neomycin resistance; fusion, CSB-PGBD3 fusion protein. Puromycin-resistant cells express untagged proteins from the bicistronic vector pIRESpuro (Clontech); hygromycin-resistant cells express FLAG-HA tagged proteins or the FLAG-HA tag alone; neomycin-resistant cells express FLAG-tagged proteins or the FLAG tag alone. Hyphens indicate transformation protocol for cell lines, antibiotic selection for transfection of genes and tags. The microarray experiments (Table S1) were performed using singly-transfected CSB-hyg and fusion-hyg pools, and a doubly-transfected CSB-hyg + fusion-neo line, all normalized for consistency to hyg alone. See Materials and methods for details.

Cell line	Source	Use in this study
CS1AN-hTERT	Newman et al. [3]
CS1AN-hTERT + eGFP-puro	Newman et al. [3]	Table 4
CS1AN-hTERT + CSB-puro	Newman et al. [3]	Table 4
UVSS1KO-SV40 + hyg	this study	Fig. 2A, Tables 4 and S1
UVSS1KO-SV40 + fusion-hyg	this study	Fig. 2A, Tables 4 and S1
UVSS1KO-SV40 + CSB-hyg	this study	Fig. 2A, Tables 4 and S1
UVSS1KO-SV40 + hyg + neo	this study	Fig. 2B,C
UVSS1KO-SV40 + hyg + fusion-neo	this study	Fig. 2B,C
UVSS1KO-SV40 + fusion-hyg + neo	this study	Fig. 2B,C
UVSS1KO-SV40 + CSB-hyg + neo	this study	Fig. 2B,C
UVSS1KO-SV40 + CSB-hyg + fusion-neo	this study	Fig. 2B,C, Tables 4 and S1