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. Author manuscript; available in PMC: 2013 Apr 27.
Published in final edited form as: Mol Cell. 2012 Mar 22;46(2):125–135. doi: 10.1016/j.molcel.2012.02.015

Figure 5. Genomic instability in metaphases from cells treated overnight with drugs to induce DNA interstrand cross-links (ICLs).

Figure 5

(A) Genomic instability in B cells from WT, FANCD2−/− and FANCD2−/−53BP1−/− mice treated overnight with 5µM cisplatin. (B) Genomic instability in B cells from WT, FANCD2−/− and FANCD2−/−53BP1−/− mice treated overnight with 250 nM mitomyin C (MMC). (C) Total genomic aberrations in metaphases from FANCD2−/− and FANCD2−/− Ku80−/− mouse embryonic fibroblast cells after overnight treatment with 5µM cisplatin. (D) Genomic instability in metaphases from FANCD2−/− and FANCD2−/−Ku80−/− after overnight treatment with 250nM MMC. (E) Colony forming assay in WT, FANCD2−/− and FANCD2−/−Ku80−/− MEFs treated with cisplatin. (F) Colony forming assay WT, FANCD2−/− and FANCD2,Ku80 double-knockout MEFs treated with mitomycin C. (Mean +/− standard deviation shown.) See also Figure S5.