Figure 1. Defects in body weight and longevity in Lmna^−/− and Lmna^L530P/L530P (LmnaΔ9 mice) mice are ameliorated in homozygous Sun1 knockout Lmna^−/−Sun1^−/− and LmnaΔ9Sun1 ^−/− animals.

(A) Body weights are averages from mice with the indicated genotypes. The number (n) of animals used is indicated.

(B) Kaplan-Meier graph showing increased life span of Lmna^−/−Sun1^−/− compared to Lmna^−/− mice. Median survival of wild type or Sun1^−/− is >210 days in a 7 month follow up; Lmna^−/− mice have median survival of 41 days; Lmna^−/−Sun1^+/− mice have a median of 54 days; Lmna^−/−Sun1^−/− mice have a median of 104 days (P <0.01 comparing Lmna^−/− and Lmna^−/−Sun1^−/−).

(C) Body weights of LmnaΔ9 mice that are wild type, heterozygous, or homozygous for Sun1 deficiency. Wild type and Sun1^−/− cohorts are graphed for comparison. Values are averages+/−SEM from animals in each cohort. Number (n) of animals is indicated. (P <0.0001 comparing LmnaΔ9Sun1 ^+/+ and LmnaΔ9Sun1 ^−/−).

(D) Kaplan-Meier graph showing increased life span of LmnaΔ9Sun1 ^−/− compared to LmnaΔ9Sun1 ^+/+ mice. LmnaΔ9Sun1 ^+/− mice are also graphed. (P <0.0001 comparing LmnaΔ9Sun1 ^+/+ and LmnaΔ9Sun1 ^−/−).

(E) Cell proliferation of the indicated MEFs. Curves are averages+/−SD, representative of >3 independent isolates from embryos of the indicated genotypes.

(F) Proliferation curves of MAFs (mouse adult fibroblasts) from WT, Sun1^−/−, LmnaΔ9Sun1 ^+/+ and LmnaΔ9Sun1 ^−/− mice. MAFs were seeded at a density of 1000 cells per well. Growth was measured, and normalized cell indexes (averages+/−SD) are presented.