Fig. 7.
Effect of hypoglycemia and lactate/pyruvate substitution on the NAD(P)H biphasic response. Slices were incubated with 2.5 mmol/L glucose for 30 min before the stimulus train and were returned to 10 mmol/L glucose after 60 min of exposure to hypoglycemia. In some cases, slices were supplemented with pyruvate or lactate (20 mmol/L) during hypoglycemia. Data are the mean ± SEM of 4–13 slices. *p < 0.05 versus control (ANOVA and Tukey's multicomparison test).