Total PKMζ protein levels are elevated 28 days post-C2 hemisection. PKCζ/ι and PKMζ expression were measured by immunoblots in ventral C3–5 homogenates either ipsi- or contralateral to injury from sham (white bar) and C2 hemisected (black bar) rats. (A) One day post-injury, PKMζ expression is significantly higher on the ipsilateral versus contralateral sides following a C2HS (ipsi v. contra, p=0.012), but neither side is significantly different from sham rats. (B) Three days post-injury, there were no significant differences in PKMζ expression on either side (p>0.05). (C) 28 days post-injury, PKMζ expression is significantly higher ipsilateral to injury (C2HS v. sham, p=0.011; ipsi v. contra, p=0.020). On right, typical gels exemplify change in PKMζ, but there were no consistent changes in other aPKC isoforms.