Table 1. PCR primer and product length of target gene HIF-1α and iNOS GAPDH.

Gene	Primer series	PCR product length
Endo-reference
GAPDH	Upstream: 5'- ACC ACA GTC CAT GCC ATC AC-3'	452bp
	Downstream: 5'- TCC ACC ACC CCT GTT GCT GTA-3'
Target gene
HIF-1α	Upstream: 5'- CTG ATT GCA TCT CCA CCT TCT ACC -3'	343bp
	Downstream: 5'- TTC CAA GAA AGC GAC ATA GTA GGG -3'
iNOS	Upstream: 5'- TCC AGG AGG ACA TGC AGC AC -3'	260bp
	Downstream: 5'- TCT TGA CGC CTT CCC GC -3'