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. 2012 Feb 18;5(1):7–12. doi: 10.3980/j.issn.2222-3959.2012.01.02

Table 1. Specific sets of primers and conditions of PCR.

Primers Nucleotide sequence(5′→3′)sense/anti-sense Annealing Temperature(°C) PCR Cycles
VEGF 5′-AGCCGAGCTCATGGACGGGT-3′ 56 35
5′-GCACGCACTCCAGGGCTTCA-3′
C-Kit 5′-TCGCAGCTGGCGCGATGG-3′ 57 35
5′-AGTGCCGCTTCTGCCTGCTC-3′
CXCR4 5′-TTTGCCGACGTCAGCCAGGG-3′ 58 37
5′-GGATG ACGATGCCGGGCAGG-3′
SDF-1α 5′-TGCCCCTGCCGGTTCTTCGAG-3′ 57 35
5′-CTGTTGTTGTTCTTCAGCCGT-3′
GAPDH 5′-ACCACAGTCCATGCCATCAC-3′ 58 25
5′-TCCACCACCCTGTTGCTGTA-3′