Table 1. Specific sets of primers and conditions of PCR.
Primers | Nucleotide sequence(5′→3′)sense/anti-sense | Annealing Temperature(°C) | PCR Cycles |
VEGF | 5′-AGCCGAGCTCATGGACGGGT-3′ | 56 | 35 |
5′-GCACGCACTCCAGGGCTTCA-3′ | |||
C-Kit | 5′-TCGCAGCTGGCGCGATGG-3′ | 57 | 35 |
5′-AGTGCCGCTTCTGCCTGCTC-3′ | |||
CXCR4 | 5′-TTTGCCGACGTCAGCCAGGG-3′ | 58 | 37 |
5′-GGATG ACGATGCCGGGCAGG-3′ | |||
SDF-1α | 5′-TGCCCCTGCCGGTTCTTCGAG-3′ | 57 | 35 |
5′-CTGTTGTTGTTCTTCAGCCGT-3′ | |||
GAPDH | 5′-ACCACAGTCCATGCCATCAC-3′ | 58 | 25 |
5′-TCCACCACCCTGTTGCTGTA-3′ |