Fig. 4.

Metabolic regulators of substrate metabolism and capillary density. A: glucose transporter 4 (GLUT4), hexokinase II (HKII), and peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) from the peri-infarct region, as determined by immunoblotting. B: representative immunoblotting performed to measure GLUT4, HKII, and PGC-1α. Protein levels are normalized to actin content and are relative to the SHAM group; n = 6–8 mice/group. C: PECAM-1 immunofluorescence staining of the infarct. D: PECAM-1 immunofluorescence staining of the remote left ventricle. Vascular density determined as the %immunopositive PECAM-1 area/total tissue area. Values are relative to SHAM left ventricle; n = 9–10 mice/group. Scale bar, 50 μm. Data are means ± SE. *P < 0.05 vs. SHAM. Of note, the order presentation of the groups in B is different from the order presentation of the groups in A, C, and D. The SHAM group presented in C and D is the same data set.