Table 1.
Bacterium | Gram | Percentage of bacteria killed
|
|
---|---|---|---|
Method A | Method B | ||
S. aureus | (+) | 94 ± 4 | 94 ± 3 |
S. epidermidis | (+) | >99 | >99 |
P. aeruginosa | (−) | >99.8 | >99.8 |
E. coli | (−) | >99 | >99 |
Suspensions (106 cells per ml for the first three bacteria and 107 cells per ml for the last one) of bacteria in distilled water were sprayed on hexyl-PVP-modified glass surfaces, air dried for 2 min, and incubated under 0.7% agar in a bacterial growth medium overnight, and then the colonies were counted. The number of viable cells obtained in the same manner with commercial NH2-glass slides was used as a standard (i.e., 0% of the bacteria killed). All experiments were performed at least in quadruplicate, and the errors indicate the standard deviations. See Materials and Methods for a detailed description of methods A and B.