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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Stem Cells. 2011 Jul;29(7):1052–1063. doi: 10.1002/stem.662

Figure 1. Generation of Cynomolgus Monkey iPSC.

Figure 1

(A–B) Phase contrast images showing ESC-like morphology of CM-iPSCs on MEF. (C) High-magnification image of CM-iPSCs showing the high nucleus/cytoplasm ratio and prominent nucleoli. (D) Immunofluorescence staining for the pluripotency markers Nanog, Oct4, SSEA-4, TRA-1–60, and TRA-1–81. (E) CM-iPSC colonies stained positive for alkaline phosphatase (AP). (F) Karyotype analysis of CM-iPSCs (line 27-04, passage 10). CM-iPSCs maintained a normal 42, XY karyotype after expansion.(G) Quantitative RT-PCR showing induction of endogenous transcripts of Sox2, Oct4, Klf4, and c-Myc. Primers specific for endogenously (black) or viral exogenous (red) encoded transcripts of the four reprogramming factors. Monkey dermal fibroblasts, four days after transduction with the four retroviruses (RV-FIB), were used as a positive control for expression of the viral transgenes. Values were normalized by the averaged value of β-actin. Values are expressed as averages + SEM of 3 independent experiments. The value of hESCs (H9) was set to 1 in each experiment. Scale bar, 200 µm (A–B, D–E); 20 µm (C).