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RT-PCR detection of β3-adrenergic receptor (β3-AR) mRNA in detrusor smooth muscle (DSM) whole tissue and isolated DSM single cells. The expected size of the product was 387 bp. RT (+), reverse transcription; RT (−), no reverse transcription. Guinea pig ileal tissue was used as a positive control. Illustrated gel image is a selected representation of at least 5 independent RT-PCR experiments based on RNA extracted from 4 animals.
